Objective To analyze the sequence of Hantavirus(HV) from Zhejiang, Fujian provinces and Shanghai municipal. Methods The sequence of M segment of several HV strains from coastal areas of Zhejiang, Fujian province and Shanghai municipal were collected and analyzed by Mega 4.0 and DNAStar software, phylogenetic trees were built by neighbor joining method (NJ) and analyzed to compare the similarity of HV strains isolated from Zhejiang, Fujian provinces and Shanghai municipal. Results The phylogenetic tree revealed that the similarity of isolates in the same province is much higher than the other, meanwhile, in same province or city, the nucleotide similarity of the same region is higher than the other regions. The distributions of the isolates from closer regions share higher sequence similarity in the phylogenetic branches. The ZH53 strain of HTN isolated from Fujian province, Gou3 strain and ZJ5 strain of SEO isolated from Jiande region in Zhejiang province formed a distinct phylogenic lineage in HTNV clade and SEOV clade respectively, and these strains were different from other variants and international standard strains with the similarity of 82.7%-86.3% and 84.0%-85.3% respectively. The SEOV strains in Zhejiang were isolated from field mouse indicating the phenomenon of “host spillover”. Conclusion The sequence similarity and the phylogeny of HV in southeast costal area of Zhejiang, Fujian provinces and Shanghai is closed related to the isolated regions, indicating geographic distribution of HV.

Objective To analyze the prevalence and genotypes of Hantavirus (HV) among rodents in the epidemic areas of hemorrhagic fever with renal syndrome (HFRS) in Zhejiang province, China from 2008 to 2011. Methods Direct immunofluorescence assay and RT-PCR were used to detect the antigen and nucleic acid of HV. Mongolian gerbils and Vero-E6 cells were used to isolate viruses from the HV-positive rodents, and the M segments of HV isolates were subjected to sequence analysis. Results Apodemus agrarius was the dominant species of the rodents captured in the epidemic areas of HFRS in Zhejiang province from 2008 to 2011. Six strains of HV were isolated from the lungs of this rodent species. The sequence analysis showed that the six isolates belonged to Hantaan virus (HTNV), wherein Z521, 524, and 534 were classified as H7 subtype, and TT-27, T-43, and R88 might be classified as a new subtype. The six HV isolates showed high gene homology between them and with the strains previously isolated in Zhejiang province. Conclusion HTNV was prevalent in rodent in the epidemic areas of HFRS in Zhejiang province from 2008 to 2011.

【Abstract】 Objective To clarify the natural infection situation of rodents by Hantavirus (HV) and HV strains in Zhejiang province in 2007，and provide science evidence for the prevention and control of hemorrhagic fever with renal syndrome (HFRS)．Methods Lung tissue and serum from rodent were sampled, and IgG antibody from serum was tested by indirect immunofluorscence assay and direct immunofluorscence assay was adopted to test HV antigens from lung tissues. HV was isolated by Vero?E6 cells, and HV strains were identified by Anti?McAb．Results A total of 1129 rodents were captured in 2007．The dominant specie was Apodemus agrarius, and the positive rate of HV antigen in rodent lungs was 3.0%. The IgG antibody of 57 blood samples was positive, and the positive rate was 8.0%.  Six  strains  of  hantaan (HTN)  virus  were  isolated,  five  strains  from A.agrarius  and  one  from  Rattus norvegicus.  Conclusion There  are  natural  foci  of  HFRS  which  main  infection  sources  are A.agrarius in Zhejiang province, and HTN strain could be the main prevalence strains of HV.

【Abstract】 Objective To explore the epidemiological characteristics of hemorrhagic fever with renal syndrome(HFRS) in Zhejiang province for establishing pertinent prevention strategy. Methods Descriptive epidemiology method was applied for the analysis of epidemic situation. The antibody in the serum of HFRS patients was detected by indirect immunoflorscence method, and the HV antigen in the rodent lung was assayed by direct immunoflorscence method. Results There were 757 cases in the whole province in 2007, mainly distributed in Ningbo, Shaoxing, Taizhou, Lishui and Quzhou, accounting for about 86.79% of the total （657/757）. There were two incidence peaks in summer, winter and spring. Most of the cases were young and middle?aged peasants. Conclusion The incidence rate of HFRS in Zhejiang province has been stable in recent years, but it was still high in some counties and cities. Therefore, the prevention and control of HFRS in the main epidemic areas should be strengthened.

Objective To investigate the epidemiology of the hemorrhagic fever with renal syndrome(HFRS) and the serotype of hantavirus(HV).Methods Direct immunoflorscence assay(DFA) was adopted to detect HFRS antigen and the virus was isolated from the young Meriones unguiculata inoculated with the rat lung tissues infected by HV.The isolated virus were continuously propagated in the Vero-E6 cell and identified by DFA using Anti-HV McAb.Results Three strains of HV were isolated from the eight lung tissues infected by HV which belonged to the hantaan type.The virus titer of three strains could reach 10 ^4.75-10 ^5.25 CCID ₅₀/ml in the Vero-E6 cell.Conclusion Three strains of hantaan virus were successfully isolated from the lung tissue of rats,which could be the candidate strain for HFRS vaccine.